Heterologous Protein Production
Through systems biology analysis of the protein secretion pathway in Saccharomyces cerevisiae we are aiming at identifying rate controlling steps in protein biosynthesis and secretion. We are using different proteins to evaluating the secretion pathway, e.g. insulin and a-amylase, and we are also using different expression systems for varying the gene expression level.
People:
Dina Petranovic, Assistant Professor (collaborator)
Jin Hou, Post Doc
José L. Martínez, Post Doc
Zihe Liu, PhD student
Lifang Liu, PhD student
Production of biofuels
We are working on the development of yeast cell factories for the production of novel, high valuable biofuels. The research involves a combination of synthetic biology where complete heterologous pathways are being reconstructed and metabolic engineering of the central carbon metabolism for redirection of carbon fluxes to the desired products.
People:
Verena Siewers, Project Leader
Shuobo Shi, Post Doc
Anastasia Krivoruchko, Post Doc
António Roldão, Post Doc
Klaas Buijs, PhD student
Bouke de Jong, PhD student
Juan Octavio Valle, PhD student
Yiming Zhang, PhD student
Production of chemicals
Fungal cell factories are being developed for the production of a range of different chemicals, including high-value chemicals to be used as perfumes and fine fragrances and commodity chemicals to be used for sustainable production of polymers. The research involves a combination of metabolic engineering of the central carbon metabolism for redirection of carbon fluxes to the desired products and systems biological characterization of metabolism.
People:
Verena Siewers, Project Leader
Yun Chen, Post Doc
Il-Kwon Kim, Post Doc
Marina Sanchez Martínez, Post Doc
Siavash Partow, PhD student
Gionata Scalcinati, PhD student
Christoph Knuf, PhD student
Marta Papini, PhD student
Jichen Bao, MSc student
Stefan Tippmann, visiting MSc student
Synthetic Biology Tools
We are developing novel synthetic biology tools for the yeast Saccharomyces cerevisiae. This involves characterization of promoters, generation of synthetic promoters, tools for rapid reconstruction of synthetic pathways and novel vectors for gene expression.
People:
Verena Siewers, Project Leader
Il-Kwon Kim, Post Doc
Kanokarn Kocharin, PhD student
Adnan Kadic, MSc student
Yuan Yuan, MSc student
Central carbon metabolism
We are using the yeast Saccharomyces cerevisiae as a model organism for studying the regulation of carbon and energy metabolism, and how this is linked to lipid metabolism and ageing. Our focus is on how the protein kinases Snf1 and Tor1 interacts and integrates nutrient sensing signals. The research involves systems biology studies of signal transduction pathways.
People:
Rahul Kumar, Post Doc
Sakda Khoomrung, Post Doc
Jie Zhang, PhD student
Kuk-ki Hong, PhD student
Pramote Chumnapuen, PhD student
Metabolic modeling
Genome-scale metabolic models are being developing for microorganisms, human and mouse cells. Through the development of novel computational tools we are advancing the reconstruction process. We are also developing novel simulation tools and integrative analysis tools that allows for integrative analysis of omics data.
People:
Sergio Bordel Velasco, Assistant Professor
Intawat Nookaew, Project Leader
Luis Caspeta, Post Doc
Roberto Olivares-Hernandez, PhD student
Saeed Shoaie, PhD student
Rasmus Ågren, PhD student
Tobias Österlund, PhD student
Joachim Almquist, PhD student
Kaisa Thorell, PhD student
Amir Feizi, MSc student
Systems medicine
Through collaborations we are involved in a number of projects where we are using our metabolic models for integrative analysis of transcriptome data. Our focus is on metabolic diseases such as diabetes, arteriosclerosis and hypertension, but we are also analyzing the effect of cancer development on metabolism. We are also looking into modeling the metabolism of the gut microbiome.
People:
Sergio Bordel Velasco, Assistant Professor
Intawat Nookaew, Project Leader
Dina Petranovic, Assistant Professor (collaborator)
Adil Mardinoglu, Post Doc
Fredrik Karlsson, PhD student
Natapol Pornputtapong, PhD student
Kemal Sanli, MSc student
Leif Väramo, MSc student
