António Roldão, PhD Department of Chemical and Biological Engineering, Chalmers University of Technology, Kemivägen 10, SE-412 96 Göteborg, Sweden Phone: +46(0)31 772 3847 Fax: +46(0)31 772 3801 E-mail: antnio [at] chalmers.se Office: Room 3057A     Education and degrees 2003 B.S. in Chemical Engineering, FCT-UNL, Portugal 2010 Ph.D. in Engineering and Technology Sciences - Systems Biology, ITQB-UNL, Portugal Employments 2004-2005 Junior Researcher, IBET & ITQB-UNL, Portugal 2006-2010 PhD student, Animal Cell Technology Unit, ITQB-UNL, Portugal 2009 Bioprocess Engineer, GenIBET Biopharmaceuticals, Portugal 2010- Post doc, Department of Chemical and Biological Engineering, Chalmers Publications, Patents & Presentations Publications 2005 Helena L.A. Vieira, Catarina Estêvão, António Roldão, Cristina C. Peixoto, Marcos F.Q. Sousa, Pedro E. Cruz, Manuel J.T. Carrondo and Paula M. Alves. Triple layered rotavirus VLP production: Kinetics of vector replication, mRNA stability and recombinant protein production. Journal of Biotechnology, 120 (1): 72-82. 2006 Teresa Rodrigues, Andreia Carvalho, António Roldão, Manuel J.T. Carrondo, Paula M. Alves, Pedro E. Cruz. Screening anion-exchange chromatographic matrices for isolation of onco-retroviral vectors, Journal of Chromatography B, 837(1-2): 59-68.   Cruz PE, Silva AC, Roldão A, Carmo M, Carrondo MJ, Alves PM. Screening of novel excipients for improving the stability of retroviral and adenoviral vectors, Biotechnology Progress, 22(2): 568-76.   Coroadinha AS, Ribeiro J, Roldão A, Cruz PE, Alves PM, Merten OW, Carrondo MJ. Effect of medium sugar source on the production of retroviral vectors for gene therapy, Biotechnology & Bioengineering, 94(1): 24-36.   António Roldão, Helena L. A. Vieira, Paula M. Alves, Rui Oliveira, Manuel J.T. Carrondo. Intracellular dynamics in Rotavirus-like particles production: Evaluation of multigene and monocistronic infection strategies. Process Biochemistry 41, 2188-2199. 2007 António Roldão, Helena L.A. Vieira, Annie Charpilienne, Didier Poncet, Polly Roy, Manuel J.T. Carrondo, Paula M. Alves and Rui Oliveira. Modelling Rotavirus-Like Particles production in a baculovirus expression vector system: infection kinetics, baculovirus DNA replication, mRNA synthesis and protein production. Journal of Biotechnology, 128(4): 875-894.   Isabel Marcelino, Nathalie Vachiéry, Ana Isabel Amaral, António Roldão, Thierry Lefrançois, Manuel J.T. Carrondo, Paula M. Alves, Dominique Martinez. Effect of the purification process and the storage conditions on the efficacy of an inactivated vaccine against heartwater, Vaccine, 25(26): 4903-4913. 2008 António Roldão, Manuel J.T. Carrondo, Paula M. Alves and Rui Oliveira. Stochastic simulation of protein expression in the baculovirus/insect cells system. Computers and Chemical Engineering, 32(1-2): 68-77. 2009 António Roldão, Rui Oliveira, Manuel J. T. Carrondo and Paula M. Alves. Error Assessment in Recombinant Baculovirus Titration: Evaluation of Different Methods. Journal of Virological Methods, 159(1): 69-80. 2010 António Roldão, Maria Candida M. Mellado, Leda R. Castilho, Manuel J. T. Carrondo, Paula M. Alves. Virus-Like Particles in Vaccine Development. Expert Review of Vaccines, 9(10): 1149-1176. 2011 António Roldão, Tiago Vicente, Cristina Peixoto, Manuel J. T. Carrondo and Paula M. Alves. Quality control for baculovirus-based products. Journal of Invertebrate Pathology (In press). Book chapters 2011 António Roldão, Ana Carina Silva, Maria Candida M. Mellado, Paula M. Alves, Manuel J. T. Carrondo. Viruses and virus-like particles in biotechnology: fundamentals and applications. In: Moo-Young and Butler Eds. Comprehensive Biotechnology, 2nd edition, Volume 1: Scientific Fundamentals in Biotechnology, Elsevier, Oxford (In press). Talks   2005 António Roldão, Helena L.A. Vieira, Manuel J.T. Carrondo, Paula M. Alves and Rui Oliveira. Modeling and Control of Rotavirus Virus-Like Particle Production: Baculovirus DNA Replication, mRNA Synthesis and Protein Production. 9th International Chemical Engineering Conference, September 21st – 23rd 2005, Coimbra, Portugal. 2006 António Roldão, Helena L. A. Vieira, Manuel J.T. Carrondo, Paula M. Alves and R. Oliveira. Rotavirus-Like Particle Production: Simulation of Protein Production and Particle Assembly. ESCAPE-16 + PSE 2006, July 9th – 13rd, Garmisch-Partenkirchen, Germany. 2008 António Roldão, Manuel J.T. Carrondo, Paula M. Alves and Rui Oliveira. Stochastic Modelling of the Baculovirus/Insect Cells System: Prediction of Recombinant Protein Expression. 10th International Chemical Engineering Conference, September 4th – 6th, Braga, Portugal. 2008 António Roldão, Manuel J.T. Carrondo, Paula M. Alves and Rui Oliveira. Predicting Recombinant Protein Expression in the Baculovirus/Insect Cells System: A Stochastic/Structured Modeling Approach. ESF 2nd Functional Dynamics Conference, September 15th – 18th, Rotenburg ob der Tauber, Germany. Poster presentations 2006 António Roldão, Helena L.A. Vieira, Catarina Estêvão, Manuel J.T. Carrondo, Paula M. Alves and Rui Oliveira. Modeling Rotavirus VLPs Production: Infection, Gene expression and VLPs assembly, Cell Culture Engineering X, April 23rd – 28th, Whistler, Canada. 2008 António Roldão, Manuel J. T. Carrondo, Paula M. Alves and Rui Oliveira. Rotavirus-Like Particle Production: Mathematical Modeling Rational Approach for Process Development, Vaccine Technology II, June 1st – 6th, Albufeira, Portugal. 2009 António Roldão, Manuel J. T. Carrondo, Paula M. Alves and Rui Oliveira. In Silico Model of the AcMNPV/Sf-9 System for Viral Protein Expression and RLP Assembly: a Bottom-Up System Biology Approach, 10th International Conference on Systems Biology, 30th August – 4th September, California, USA.   António Roldão, Manuel J. T. Carrondo, Paula M. Alves and Rui Oliveira. In Silico Model of the AcMNPV/Sf-9 System for Viral Protein Expression and RLP Assembly: a Bottom-Up System Biology Approach, 1st summer school of the IMPRS Magdeburg: The theory of process engineering, Max-Planck Institute for Dynamics of Complex Technical Systems & Otto von Guericke University Magdeburg, September 28th – October 2nd, Magdeburg, Germany. Teaching and Tutoring Experience 2006-2010 Instructed undergraduate, B.Sc., PhD students and Post-docs 2010 Theoretical/practical classes within the scope of graduation and master’s programs Grants, Awards and Prizes 2003 PRODEP III, Portugal 2006 PhD grant by Fundação para a Ciência e Tecnologia, Portugal 2006 Grant to attend the Cell Culture Engineering X conference, Canada 2008 Grant to attend the Vaccine technology II conference, Portugal 2008 European Science Foundation grant to attend the ESF 2nd Functional Dynamics Conference, Germany 2009 Fundação Calouste Gulbenkian grant to attend the ICSB10, USA Organization and management 2004 Organizing Committee of 3rd Recombinant Protein Production Meeting, Tavira, Portugal 2008 Participation in the organization of Vaccine Technology II, Albufeira, Portugal Personal text My domain of specialization is bioprocess development and dynamic mathematical modelling, with emphasis on the baculovirus/insect cell system. My research interests include bottom-up Systems Biology approaches to tackle complex biological systems and process optimization and control via mathematical model-based strategies. I have recently joined Dr. Nielsen’s lab to become an expert in Systems and Synthetic Biology. Currently, I am working in Synthetic Biology and its application for developing efficient and robust production platforms of high-value biofuels.