Keith EJ Tyo, PhD Department of Chemical and Biological Engineering, Chalmers University of Technology, Kemivägen 10, SE-412 96 Göteborg, Sweden Phone: +46(0)31 772 3846 Fax: +46(0)31 772 3801 E-mail: keitht [at] chalmers.se Office: Room 3056B     Education and degrees 2003 M.S. in Chemical Engineering Practice, Massachusetts Institute of Technology, USA 2008 Ph.D. in Chemical Engineering, Massachusetts Institute of Technology, USA Employments 2000-2001 Summer Research Intern, Dow Chemicals Company, USA 2002-2008 Research Assistant, Department of Chemical Engineering, MIT, USA 2008- Post doc, Department of Chemical and Biological Engineering, Chalmers Publications, Patents & Presentations Publications 2005 R.M. Raab, K.E. Tyo, G.N. Stephanopoulos. “Metabolic Engineering.”  Advances in Biochemical Engineering/Biotechnology, Springer-Verlag GmbH, 2005, 100,1. 2006 K.E. Tyo, H. Zhou, G.N. Stephanopoulos.  “High-Throughput Screen for Poly-3-Hydroxybutyrate in Escherichia coli and Synechocystis sp. Strain PCC6803.” Applied and Environmental Microbiology, 2006, 72(5), 3412. 2007 K.E. Tyo, H.S. Alper, G.N. Stephanopoulos.  “Expanding the metabolic engineering toolbox: more options to engineer cells.” Trends in Biotechnology, 2007, 25(3), 132.   K.E. Tyo, B.L. Wang, G.N. Stephanopoulos.  “Metabolic Engineering.” McGraw-Hill 2007 Yearbook of Science & Technology.  McGraw-Hill, 2007, 144. 2008 H. Yu, K.E. Tyo, H. Alper, D. Klein-Marcuschamer, and G. Stephanopoulos.   “A high-throughput screen for hyaluronic acid accumulation in recombinant Escherichia coli transformed by libraries of engineered sigma factors.”  Biotechnology and Bioengineering, 2008, 101(4), 788.   P.K. Ajikumar, K.E. Tyo, S. Carlsen, O. Mucha, T.H. Phon, G.N. Stephanopoulos. “Terpenoids: Biosynthesis of a versatile therapeutic class through engineered microorganisms.” Molecular Pharmaceutics, 2008, 5(2), 167. 2009 K.E. Tyo, P.K. Ajikumaran, G.N. Stephanopoulos. “Stabilized gene duplication enables long-term selection-free heterologous pathway expression.” Nature Biotechnology, 2009, 27, 760.   K.E. Tyo, Y.S. Jin, F.A. Espinoza, G.N. Stephanopoulos. “Inverse Metabolic Engineering of Synechocystis PCC 6803 for Poly-3-hydroxybutyrate Accumulation.” Biotechnology Progress. 2009 25(5), 1236.   K.E. Tyo, C. Fischer, F. Simeon, G. Stephanopoulos.  “Analysis of polyhydroxybutyrate flux limitations by systematic genetic and metabolic perturbations.” Metabolic Engineering.  (accepted) Presentations 2005 K.E. Tyo, H. Zhou, H.S. Alper, G.N. Stephanopoulos.  “A High-Throughput Screen for Poly-3-hydroxybutyrate Accumulation for Inverse Metabolic Engineering.” [Oral]  American Institute of Chemical Engineers Annual Meeting in Cincinnati, OH.  November 2005.   K.E. Tyo, G.N. Stephanopoulos.  “Combinatorial methods for identifying gene knock-out targets for phenotypic improvement in Synechocystis PCC 6803.”  [Oral] American Chemical Society Annual Meeting in San Diego, CA. March 2005.   K.E. Tyo, G.N. Stephanopoulos.  “Inverse metabolic engineering of Synechocystis PCC 6803 for improved growth rate and poly-3-hydroxybutyrate production.” [Poster] Singapore-MIT Alliance Symposium in Singapore. January 2005. 2007 K.E. Tyo, G. N. Stephanopoulos.  “Growth-phase accumulation of poly-3-hydroxybutyrate in Escherichia coli: A prospectus for continuous production.” [Oral] American Chemical Society Annual Meeting in Boston, MA. August 2007. 2009 K.E. Tyo, P.K. Ajikumaran, G.N. Stephanopoulos.  “Chemically Induced Chromosomal Evolution: A preferable alternative to plasmids for high copy, long-term, antibiotic-free expression.”  [Poster]  International Conference on Biomolecular Engineering in Santa Barbara, CA.  January 2009.   Z. Liu, K. Tyo. D. Petranovic, J. Nielsen. “Recombinant Insulin Production by Saccharomyces cerevisiae.” [Poster] FEMS 3rd Conference of European Microbiologists.  July 2009.   K. Tyo, D. Petranovic, J. Nielsen. “Biological Networking of the Yeast Unfolded Protein Response.” [Oral] 27th International Specialized Symposium on Yeast.  August 2009.   Z. Liu, K. Tyo, D. Petranovic, J. Nielsen. “Heterologous protein production in Saccharomyces cerevisiae.” [Poster] 27th International Specialized Symposium on Yeast.  August 2009.   K.E. Tyo, P. Ajikumar, G. Stephanopoulos.  “Chemically Inducible Chromosomal Evolution (CIChE): Increasing Genetic Stability by Avoiding the Pitfalls of Plasmids.”  [Oral]  American Institute of Chemical Engineers Annual Meeting in Nashville, TN.  November 2009.   K.E. Tyo, C. Fischer, G. Stephanopoulos. “Genetic and Metabolic Perturbation Analysis of the Polyhydroxybutyrate Pathway.”  [Oral]  American Institute of Chemical Engineers Annual Meeting in Nashville, TN.  November 2009.   K.E. Tyo, J. Nielsen, G. Stephanopoulos.  “Meeting in the Middle: Top-Down and Bottom-up Approaches to Metabolic Engineering.”  [Poster]  American Institute of Chemical Engineers Annual Meeting in Nashville, TN.  November 2009. Teaching and Tutoring Experience 2004-2006 Undergraduate Research Opportunities Program, Supervisor. MIT, USA (3 students) 2004 Heat and Mass Transport, Teaching Assistant. MIT, USA 2006 Chemical Engineering Project Lab, Consultant.  MIT, USA 2009-2010 Research Ethics Grants, Awards and Prizes 2001 Haslam Presidential Fellow (MIT) 2003 Rosemary Wojtowicz Fellow (MIT) 2008 NIH Kirschstein National Research Service Award Fellow 2009 International Conference on Biomolecular Engineering,  Poster Award - 2nd prize (SBE)   Young Scientist Meeting Grant (FEMS) Project & Objectives Current Project: The unfolded protein response (UPR) is a large-scale, coordinated response to correct ER and secretion problems in eukaryotes.  We are mapping the biological information flow of the UPR through the many genes involved in the response to achieve an understanding and identify targets for altering the response.  Biological interaction networks combined with X-omics data will be used to build this map.  Using the map, important biological molecules and interactions can be targeted to improve protein production and, in humans, identify targets to prevent/treat aggregated-protein related disease, such as Alzheimer’s, Type 2 diabetes, and apoptosis-resistant, uncontrolled protein production in certain cancers. Objectives: My career goals are to obtain a faculty position at a research university.  I would like to manage research programs in metabolic engineering and synthetic biology that address difficulties in producing therapeutics in microbes and development of microbe-based detection tools for the molecular diagnosis of disease.  The fundamental issues of diagnosis and treatment directly affect the rising health care costs in the United States, as well as the availability for treatment in many developing countries.  By addressing these topics in an academic research setting, I will be able to make direct impact on these problems while training like-minded individuals in the effective application of biological tools to health care issues.